1OJJ
Anatomy of glycosynthesis: Structure and kinetics of the Humicola insolens Cel7BE197A and E197S glycosynthase mutants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.364, 74.746, 85.820 |
Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
Resolution | 15.000 * - 1.400 |
R-factor | 0.15 |
Rwork | 0.149 |
R-free | 0.17000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dym |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.580 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.033 | 0.300 |
Number of reflections | 157771 | |
<I/σ(I)> | 35 | 3.5 |
Completeness [%] | 99.0 | 96 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | HANGING DROPS 20MM TRIS-HCL PH7-8.5, 280 15-30% POLYETHYLENE GLYCOL 4000, pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH7-8.5 |
3 | 1 | reservoir | PEG4000 | 15-30 (%) |