1OJH
Crystal structure of NblA from PCC 7120
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.176, 95.918, 104.835 |
| Unit cell angles | 90.00, 97.05, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21700 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.478 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE (V. 2.03) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 0.460 |
| Number of reflections | 74292 | |
| <I/σ(I)> | 9.4 | 2.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS/HCL PH 8.5 10% PEG2000, 100 MM MGCL2, 15% ETHYLENGLYCOL |
