1OJH
Crystal structure of NblA from PCC 7120
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.176, 95.918, 104.835 |
Unit cell angles | 90.00, 97.05, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.21700 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.478 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE (V. 2.03) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.069 | 0.460 |
Number of reflections | 74292 | |
<I/σ(I)> | 9.4 | 2.8 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 100 MM TRIS/HCL PH 8.5 10% PEG2000, 100 MM MGCL2, 15% ETHYLENGLYCOL |