1OJ5
Crystal structure of the Nco-A1 PAS-B domain bound to the STAT6 transactivation domain LXXLL motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS M18X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 |
| Unit cell lengths | 62.100, 62.100, 73.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.200 * - 2.210 |
| R-factor | 0.17 |
| Rwork | 0.166 |
| R-free | 0.21400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.394 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.200 * | 2.250 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.089 | 0.325 |
| Total number of observations | 137915 * | |
| Number of reflections | 8138 | |
| <I/σ(I)> | 29.9 | 8.71 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 16.8 | 16.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 20 * | 20 % PEG3350, 0.2 M LICL, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 50 (mM) | pH7.0 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | protein | 19 (mg/ml) | |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | PEG3350 | 20 (%) |
