1OIO
GafD (F17c-type) Fimbrial adhesin from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.990, 70.470, 56.110 |
| Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 1.700 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.042 | 0.335 * |
| Total number of observations | 155927 * | |
| Number of reflections | 84352 | 2231 * |
| <I/σ(I)> | 25.3 | 3.5 |
| Completeness [%] | 88.4 | 42.8 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 4. * | 10% PEG 6000 100 MM HEPES PH 7.5, 5% MPD, 5% GLCNAC |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 10 (%(w/v)) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 3 | 1 | reservoir | MPD | 5 (%(v/v)) | |
| 4 | 1 | drop | protein | 10-30 (mg/ml) | |
| 5 | 1 | drop | GlcNAc | 5 (%(w/v)) |
