1OIO

GafD (F17c-type) Fimbrial adhesin from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-15
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 1 21 1
Unit cell lengths	42.990, 70.470, 56.110
Unit cell angles	90.00, 104.49, 90.00

Refinement procedure

Resolution	500.000 * - 1.700
R-factor	0.216
Rwork	0.216
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.007 *
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.042	0.335 *
Total number of observations	155927 *
Number of reflections	84352	2231 *
<I/σ(I)>	25.3	3.5
Completeness [%]	88.4	42.8
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5	4. *	10% PEG 6000 100 MM HEPES PH 7.5, 5% MPD, 5% GLCNAC

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	10 (%(w/v))
2	1	reservoir	HEPES	100 (mM)	pH7.5
3	1	reservoir	MPD	5 (%(v/v))
4	1	drop	protein	10-30 (mg/ml)
5	1	drop	GlcNAc	5 (%(w/v))