1OIL
STRUCTURE OF LIPASE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 295 |
Detector technology | DIFFRACTOMETER |
Collection date | 1992-03-21 |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.230, 47.420, 86.530 |
Unit cell angles | 90.00, 116.11, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.25500 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 24.200 * |
Data reduction software | FOURIER (SCALING) |
Data scaling software | FOURIER (SCALING) |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 8.000 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.040 * |
Number of reflections | 29375 |
Completeness [%] | 80.0 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.3 * | 22 * | Kim, K.K., (1992) J.Mol.Biol., 227, 1258. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MPD | 60-63 (%(v/v)) | |
2 | 1 | reservoir | sodium citrate | 200 (mM) | |
3 | 1 | reservoir | HEPES | 50 (mM) | |
4 | 1 | drop | MPD | 16 (%(v/v)) | |
5 | 1 | drop | sodium citrate | 100 (mM) | |
6 | 1 | drop | beta-octylglucoside | 0.36 (%(w/v)) | |
7 | 1 | drop | protein | 10 (mg/ml) | |
8 | 1 | drop | HEPES | 50 (mM) |