1OID
5'-Nucleotidase (E. coli) with an Engineered Disulfide Bridge (S228C, P513C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.496, 93.708, 82.901 |
| Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hp1 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.170 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.055 | 0.320 |
| Number of reflections | 194970 | |
| <I/σ(I)> | 11.3 | 2.08 |
| Completeness [%] | 98.4 | 90.8 |
| Redundancy | 2.78 | 2.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 26% PEG 400, 100 MM CACODYLATE PH 6.5 |
