1OI0
CRYSTAL STRUCTURE OF AF2198, A JAB1/MPN DOMAIN PROTEIN FROM ARCHAEOGLOBUS FULGIDUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.787, 87.081, 67.827 |
| Unit cell angles | 90.00, 94.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.500 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.24200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.395 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.067 | 0.338 |
| Number of reflections | 69516 | |
| <I/σ(I)> | 12.4 | 3.8 |
| Completeness [%] | 85.6 | 78.6 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | 17 * | 100MM MGCL2, 100MM TRIS (PH 7.8), 11% PEG 4000, 5 MM DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 2 (mM) | |
| 3 | 1 | drop | Tris-HCl | 10 (mM) | pH7.0 |
| 4 | 1 | reservoir | PEG4000 | 11 (%) | |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.8 |
