1OHZ

Cohesin-Dockerin complex from the cellulosome of Clostridium thermocellum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	110
Spacegroup name	P 21 3
Unit cell lengths	97.906, 97.906, 97.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.000 - 2.200
R-factor	0.21132
Rwork	0.210
R-free	0.24200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anu
RMSD bond length	0.006 *
RMSD bond angle	0.777 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.040	2.320
High resolution limit [Å]	2.200 *	2.200
Rmerge	0.072	0.309
Number of reflections	16170
<I/σ(I)>	6.9	1.9
Completeness [%]	99.8	99.8
Redundancy	11.9	12.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		2 (mM)
2	1	1		0.2 (M)
3	1	1	PEG3350	20 (%(w/v))
4	1	1	protein	6 (mg/ml)