1OHU

Structure of Caenorhabditis elegans CED-9

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	46.790, 92.050, 47.640
Unit cell angles	90.00, 99.78, 90.00

Refinement procedure

Resolution	30.000 - 2.030
R-factor	0.212
Rwork	0.212
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.089
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.030
Rmerge	0.042	0.201 *
Number of reflections	21636
<I/σ(I)>	17.7
Completeness [%]	84.1	54.2 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	285 *	10% PEG 8K ,0.1M HEPES PH7.5, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10 (%)
2	1	reservoir	HEPES	0.1 (M)	pH7.5
3	1	drop	protein	10 (mg/ml)
4	1	drop	sodium phosphate	20 (mM)
5	1	drop		50 (mM)
6	1	drop	beta-mercaptoethanol	2 (mM)