1OHP
CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANT D38N FROM PSEUDOMONAS TESTOSTERONI COMPLEXED WITH 5ALPHA-ESTRAN-3,17-DIONE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-15 |
| Detector | BRUKER PROTEUM 300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.353, 95.352, 60.402 |
| Unit cell angles | 90.00, 91.47, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.530 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.22900 |
| Structure solution method | DIRECT METHODS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.203 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.530 |
| Rmerge | 0.036 |
| Number of reflections | 67193 |
| <I/σ(I)> | 34.4 |
| Completeness [%] | 94.1 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | PEG 4K 24%, 0.2M AMMONIUM ACETATE, 0.1M SODIUM ACETATE PH 4.6 |
