1OHL
YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE PUTATIVE CYCLIC REACTION INTERMEDIATE COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-17 |
Detector | ADSC CCD |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 103.200, 103.200, 167.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.700 - 1.600 |
Rwork | 0.188 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ylv |
RMSD bond length | 0.005 |
RMSD bond angle | 0.013 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | CCP4 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.055 | 0.388 |
Number of reflections | 57568 | |
<I/σ(I)> | 21.7 | 2.5 |
Completeness [%] | 96.2 | 96.2 |
Redundancy | 7.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | AS FOR PDB ENTRY 1AW5, pH 8.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ALA | 0.2 (mM) | |
2 | 1 | drop | enzyme | 2 (mg/ml) | |
3 | 1 | reservoir | PEG6000 | 2-10 (%(w/v)) | |
4 | 1 | reservoir | Tris-HCl | 200 (mM) | pH7.0-8.0 |