1OHK

HUMAN DIHYDROFOLATE REDUCTASE, ORTHORHOMBIC (P21 21 21) CRYSTAL FORM

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	36.904, 69.191, 69.952
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.218 *
Rwork	0.218
RMSD bond length	0.017
RMSD bond angle	0.058
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.500
Number of reflections	5293
Completeness [%]	94.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	dioxane	10 (%)
2	1	reservoir	MES	0.1 (M)
3	1	reservoir	ammonium sulfate	1.6 (M)