1OHH

BOVINE MITOCHONDRIAL F1-ATPASE complexed with the inhibitor protein IF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	1999-02-18
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	272.300, 107.200, 152.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.500 - 2.800
R-factor	0.232
Rwork	0.232
R-free	0.27700 *
Structure solution method	MIR
Starting model (for MR)	1e1q BOVINE MITOCHONDRIAL F1-ATPASE
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	2.750
High resolution limit [Å]	2.800	2.610
Rmerge	0.081 *	0.386 *
Number of reflections	109367
<I/σ(I)>	18.1	7.1
Completeness [%]	99.4 *	97.3 *
Redundancy	4.3 *	3.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.6		PROTEIN 20MG/ML IN 100MM PIPES-NAOH PH6.6, 40MM MGSO4, 0.04% NA AZIDE, 10% GLYCEROL, 0.002% PMSF. DROPS EQUAL VOLUME OF PROTEIN AND 10MM AMP-PNP, 300MM NACL, 16% PEG 4000, 5MM SPERMIDINE. BATCH METHOD., pH 6.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	2	AMP-PNP	10 (mM)
3	1	2		300 (mM)
4	1	2	PEG4000	16 (%(w/v))
5	1	2	spermidine	5 (mM)