1OHF

The refined structure of Nudaurelia capensis omega virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	287
Detector technology	FILM
Spacegroup name	P 1
Unit cell lengths	413.550, 410.220, 419.670
Unit cell angles	59.13, 58.90, 64.04

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.219
Rwork	0.219
R-free	0.22200 *
Structure solution method	OTHER
RMSD bond length	0.007 *
RMSD bond angle	25.400 *
Data reduction software	PURDUE (DATA PROCESSING PACKAGE)
Data scaling software	PURDUE (DATA PROCESSING PACKAGE)
Phasing software	PURDUE (DATA PROCESSING PACKAGE)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Rmerge	0.121
Number of reflections	2465275
Completeness [%]	51.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5 *		Cavarelli, J., (1998) Acta Crystallogr., B47, 23. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MOPS	0.075 (M)	pH7.0
2	1	reservoir	PEG8000	2 (%)
3	1	reservoir		0.25 (M)
4	1	reservoir		0.001 (M)
5	1	drop	virus	8-10 (mg/ml)
6	1	drop	sodium acetate	0.07 (M)	pH5.0