1OHE
Structure of cdc14b phosphatase with a peptide ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF |
Synchrotron site | ESRF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-15 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.760, 53.150, 64.170 |
Unit cell angles | 90.00, 117.48, 90.00 |
Refinement procedure
Resolution | 33.000 - 2.200 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.25400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.530 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.073 * | 0.240 * |
Total number of observations | 62805 * | |
Number of reflections | 17049 * | |
<I/σ(I)> | 7.1 | 2.9 |
Completeness [%] | 97.2 | 92.2 |
Redundancy | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | 30% PEG 8000, 0.1 M ZINC ACETATE PH 8.0, 20 OC HANGING DROP VAPOUR DIFFUSION |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | Tris-acetate | 100 (mM) | pH8.0 |
3 | 1 | reservoir | PEG4000 | 30 (%) | |
4 | 1 | reservoir | 0.2 (M) | ||
5 | 1 | reservoir | dithiothreitol | 7 (mM) |