1OHD
structure of cdc14 in complex with tungstate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF |
Synchrotron site | ESRF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-15 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.750, 51.950, 64.998 |
Unit cell angles | 90.00, 118.57, 90.00 |
Refinement procedure
Resolution | 28.660 - 2.600 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.28000 * |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.660 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.119 | 0.331 |
Total number of observations | 30373 * | |
Number of reflections | 10480 | |
<I/σ(I)> | 6.8 | |
Completeness [%] | 99.5 | 99.6 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | 30% PEG 8000, 0.1 M ZINC ACETATE PH 8.0, 20 OC, HANGING DROP VAPOUR DIFFUSION |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | Tris-acetate | 100 (mM) | pH8.0 |
3 | 1 | reservoir | PEG4000 | 30 (%) | |
4 | 1 | reservoir | 0.2 (M) | ||
5 | 1 | reservoir | dithiothreitol | 7 (mM) |