1OH3
E78R mutant of a carbohydrate binding module family 29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.671, 56.549, 129.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gwm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.488 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.631 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.053 | 0.471 |
| Number of reflections | 42994 | |
| <I/σ(I)> | 16 | 1.8 |
| Completeness [%] | 92.6 | 68.4 |
| Redundancy | 3.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 25 % PEG 3350 15 % ETHYLENEGLYCOL, 0.1 M CALCIUM CHLORIDE 5 MM CELLOHEXAOASE, pH 6.00 |
