1OH2
Sucrose-Specific Porin, with Bound Sucrose Molecules
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SCHNEIDER |
Temperature [K] | 287 |
Detector | STOE |
Spacegroup name | P 32 |
Unit cell lengths | 111.800, 111.800, 147.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 100.000 - 2.400 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aos |
RMSD bond length | 0.011 |
RMSD bond angle | 1.730 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 3.000 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.054 | |
Number of reflections | 65815 | |
<I/σ(I)> | 5 | 2 |
Completeness [%] | 80.7 | 84 |
Redundancy | 2.74 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | PROTEIN WAS CRYSTALLIZED BY VAPOR DIFFUSION USING THE SITTING-DROP METHOD. THE DROP CONTAINED 5-7 MG/ML PROTEIN, 20 MM TRIS/CL AT PH 7.7, 100MM LICL, 20MM MGSO4, 1.2% BETA-D-OCTYLGLUCOPYRANOSIDE AND 6-9% PEG-2000. THE CONCENTRATION OF PEG IN THE RESERVOIR WAS 12-15%. 2M SUCROSE WAS ADDED TO THE DROP FOR COCRYSTALLIZATION. |