1OGW
Synthetic Ubiquitin with fluoro-Leu at 50 and 67
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1999-05-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.840, 42.770, 28.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.320 |
R-factor | 0.222 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubi |
RMSD bond length | 0.020 |
RMSD bond angle | 0.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXL-97 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 6.000 | 1.320 |
High resolution limit [Å] | 1.320 | 1.310 |
Rmerge | 0.110 | 0.540 |
Total number of observations | 157103 * | |
Number of reflections | 14305 * | |
<I/σ(I)> | 10 | 2.3 |
Completeness [%] | 89.9 | 81.4 |
Redundancy | 4.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | other * | 5.6 | Love, S.G., (1997) Biochem. J., 323, 727. * |