1OGT

CRYSTAL STRUCTURE OF HLA-B*2705 COMPLEXED WITH THE VASOACTIVE INTESTINAL PEPTIDE TYPE 1 RECEPTOR (VIPR) PEPTIDE (RESIDUES 400-408)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-11-25
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	51.259, 81.777, 65.617
Unit cell angles	90.00, 107.61, 90.00

Refinement procedure

Resolution	50.000 * - 1.470
R-factor	0.131
Rwork	0.128
R-free	0.17800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1of2
RMSD bond length	0.010 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.470	1.470
Rmerge	0.078	0.314
Number of reflections	84476	8185 *
<I/σ(I)>	13.3	2.2
Completeness [%]	97.6	95.2
Redundancy	4.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	291	TRIS PH 8.0, 16% PEG 8000, HANGING DROP, TEMPERATURE 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH8.0
2	1	reservoir	PEG8000	16 (%)
3	1	drop	protein	20 (mg/ml)
4	1	drop	Tris-HCl	10 (mM)	pH7.5
5	1	drop		150 (mM)