1OGT
CRYSTAL STRUCTURE OF HLA-B*2705 COMPLEXED WITH THE VASOACTIVE INTESTINAL PEPTIDE TYPE 1 RECEPTOR (VIPR) PEPTIDE (RESIDUES 400-408)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.259, 81.777, 65.617 |
| Unit cell angles | 90.00, 107.61, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.470 |
| R-factor | 0.131 |
| Rwork | 0.128 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1of2 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.078 | 0.314 |
| Number of reflections | 84476 | 8185 * |
| <I/σ(I)> | 13.3 | 2.2 |
| Completeness [%] | 97.6 | 95.2 |
| Redundancy | 4.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 | TRIS PH 8.0, 16% PEG 8000, HANGING DROP, TEMPERATURE 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
| 2 | 1 | reservoir | PEG8000 | 16 (%) | |
| 3 | 1 | drop | protein | 20 (mg/ml) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 5 | 1 | drop | 150 (mM) |
