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1OGP

The crystal structure of plant sulfite oxidase provides insight into sulfite oxidation in plants and animals

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 14-BM-C
Synchrotron siteAPS
Beamline14-BM-C
Temperature [K]100
Detector technologyCCD
Collection date2002-10-15
DetectorADSC CCD
Spacegroup nameC 2 2 2
Unit cell lengths222.918, 351.271, 158.282
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.000

*

- 2.600
R-factor0.237
Rwork0.235
R-free0.26600
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1sox
RMSD bond length0.034
RMSD bond angle2.614
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareMOLREP
Refinement softwareREFMAC (5.1.24)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.640
High resolution limit [Å]2.600

*

2.550
Rmerge0.1100.730

*

Total number of observations1177360

*

Number of reflections199819
<I/σ(I)>21.62.03
Completeness [%]99.599.5
Redundancy5.95.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

9.218

*

5-12% JEFFAMINE 600, 50 MM TRIS/HCL, 10 MM CSCL, PH 9.2
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein15-60 (mg/ml)
21reservoirJeffamine6005-12 (%)
31reservoirTris-HCl50 (mM)
41reservoirCsCl10 (mM)pH9.2

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PDB entries from 2024-11-06

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