1OGO
Dex49A from Penicillium minioluteum complex with isomaltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2002-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 115.405, 103.773, 49.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.700 * - 1.650 |
| Rwork | 0.188 |
| R-free | 0.21500 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.082 | 0.377 |
| Number of reflections | 71784 | |
| <I/σ(I)> | 7.7 | 2.4 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | 293 * | Larsson, A.M., (2002) Acta Crystallogr., D58, 346. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 0.1 (M) | pH5.5 |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | reservoir | PEG5000 MME | 20 (%) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.5 |
| 7 | 1 | reservoir | 0.1 (M) | ||
| 8 | 1 | reservoir | 10 (mM) |
