1OGM
Dex49A from Penicillium minioluteum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2000-12-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 103.670, 115.516, 50.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.300 * - 1.800 |
| Rwork | 0.142 |
| R-free | 0.16500 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | 0.409 |
| Number of reflections | 56323 | |
| <I/σ(I)> | 26 | 5.2 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | 293 * | Larsson, A.M., (2002) Acta Crystallogr., D58, 346. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 0.1 (M) | pH5.5 |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | reservoir | PEG5000 MME | 20 (%) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.5 |
| 7 | 1 | reservoir | 0.1 (M) | ||
| 8 | 1 | reservoir | 10 (mM) |
