1OGL
The crystal structure of native Trypanosoma cruzi dUTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 136.434, 136.434, 68.705 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 * - 2.400 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.26200 * |
| Structure solution method | MAD |
| Starting model (for MR) | LOW RESOLUTION MAD STRUCTURE |
| RMSD bond length | 0.030 * |
| RMSD bond angle | 2.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.051 * | 0.411 * |
| Number of reflections | 14827 | |
| <I/σ(I)> | 32.4 | 4.1 |
| Completeness [%] | 97.5 | 88.4 |
| Redundancy | 7.2 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.6 | 290 * | Harkiolaki, M., (2001) Acta Cryst., D57, 915. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 MME | 15 (%) | |
| 2 | 1 | reservoir | 0.10 (M) | ||
| 3 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.6 |
| 4 | 1 | drop | protein | 20 (mg/ml) |
