1OGF
The Structure of Bacillus subtilis RbsD complexed with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 295 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.314, 106.222, 82.648 |
| Unit cell angles | 90.00, 128.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25000 |
| Structure solution method | DIRECT METHODS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.306 |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 36109 |
| Completeness [%] | 98.3 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.4 |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 20 (%) | |
| 6 | 1 | reservoir | isopropyl | 19 (%) | |
| 7 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
