1OGE

The Structure of Bacillus subtilis RbsD complexed with Ribose 5-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	295
Spacegroup name	C 1 2 1
Unit cell lengths	123.807, 108.556, 83.532
Unit cell angles	90.00, 128.72, 90.00

Refinement procedure

Resolution	30.000 - 2.050
R-factor	0.194
Rwork	0.194
R-free	0.22700
Structure solution method	DIRECT METHODS
RMSD bond length	0.006
RMSD bond angle	1.268
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.050
Rmerge	0.048 *	0.225 *
Total number of observations	236173 *
Number of reflections	48339
Completeness [%]	90.3	80.7 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *		pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.4
3	1	drop		0.2 (M)
4	1	drop	dithiothreitol	2 (mM)
5	1	reservoir	PEG4000	20 (%)
6	1	reservoir	isopropyl	19 (%)
7	1	reservoir	sodium cacodylate	100 (mM)	pH6.5