1OGD

The Structure of Bacillus subtilis RbsD complexed with D-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	295
Spacegroup name	C 1 2 1
Unit cell lengths	123.515, 108.656, 83.308
Unit cell angles	90.00, 128.68, 90.00

Refinement procedure

Resolution	30.000 - 1.950
R-factor	0.198
Rwork	0.198
R-free	0.21100
Structure solution method	DIRECT METHODS
RMSD bond length	0.005
RMSD bond angle	1.342
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.950
Rmerge	0.067 *	0.362 *
Total number of observations	295120 *
Number of reflections	62406
Completeness [%]	97.6	92.8 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *		pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.4
3	1	drop		0.2 (M)
4	1	drop	dithiothreitol	2 (mM)
5	1	reservoir	PEG4000	20 (%)
6	1	reservoir	isopropyl	19 (%)
7	1	reservoir	sodium cacodylate	100 (mM)	pH6.5