1OG1

CRYSTAL STRUCTURE OF THE EUCARYOTIC MONO-ADP-RIBOSYLTRANSFERASE ART2.2 IN COMPLEX WITH TAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-03
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	81.419, 81.419, 77.265
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.202
Rwork	0.202
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gy0
RMSD bond length	0.014
RMSD bond angle	1.720 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.020
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.404 *
Number of reflections	20469	698 *
<I/σ(I)>	20.3	2.8
Completeness [%]	99.7	100
Redundancy	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	8.3		Mueller-Dieckmann., (2002) Acta Cryst., D58, 1211. *