1OFZ
Crystal structure of fungal lectin : six-bladed beta-propeller fold and novel fucose recognition mode for aleuria aurantia lectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.969, 86.406, 77.849 |
Unit cell angles | 90.00, 90.62, 90.00 |
Refinement procedure
Resolution | 19.960 - 1.500 |
Rwork | 0.144 |
R-free | 0.17900 |
Structure solution method | OTHER |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.556 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.960 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.049 | 0.374 |
Total number of observations | 534572 * | |
Number of reflections | 94102 * | |
<I/σ(I)> | 8.4 | 18.4 |
Completeness [%] | 96.8 | 96.8 |
Redundancy | 3.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 20 * | 2UL BUFFER (0.1 M SODIUM CACODYLATE PH 6.5, 0.2M MAGNESIUM ACETATE TETRAHYDRATE, 20% PEG 8000) PLUS 2UL OF LECTIN PROTEIN AT 10 MG/ML AND L-FUCOSE AT 137 UG/ML |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
2 | 1 | reservoir | magnesium acetate tetrahydrate | 0.2 (M) | |
3 | 1 | reservoir | PEG8000 | 20 (%) | |
4 | 1 | drop | protein | 10 (mg/ml) |