1OFU
Crystal structure of SulA:FtsZ from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.590, 75.410, 241.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.2159 |
| Rwork | 0.216 |
| R-free | 0.25500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MAD MODEL FROM C2 CRYSTAL FORM |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.261 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.096 | 0.334 |
| Number of reflections | 60044 | |
| <I/σ(I)> | 11.4 | 3.7 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.8 * | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 19 * | pH 5.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 200 (mM) | ||
| 10 | 1 | reservoir | Tris | 0.1 (M) | pH7.5 |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | GTP | 0.1 (mM) | |
| 6 | 1 | drop | 1 (mM) | pH7.5 | |
| 7 | 1 | reservoir | PEG20000 | 7.5 (%) | |
| 8 | 1 | reservoir | PEG550 MME | 7.5 (%) | |
| 9 | 1 | reservoir | sodium formate | 80 (mM) |
