1OFS
Pea lectin-sucrose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.831, 60.803, 136.087 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.860 - 1.800 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20657 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1LTN |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.480 | 0.207 |
| Number of reflections | 38608 | |
| <I/σ(I)> | 22.8 | 5.53 |
| Completeness [%] | 98.1 | 89.4 |
| Redundancy | 3.57 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 12% (W/V) PEG 20K, 8% (V/V) ETOH, 40 MM SUCROSE; CRYOSOLUTION: 0.1 M MES PH 6.5, 17% PEG 20K, 60% (V/V) ETOH, 25 MM SUCROSE |
