1OFR
CRYSTAL STRUCTURE OF THE TYROSINE-REGULATED 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE SYNTHASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH PHENYLALANINE AND MANGANESE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.287, 94.034, 105.121 |
| Unit cell angles | 65.14, 85.68, 75.73 |
Refinement procedure
| Resolution | 37.200 - 2.700 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MOLECULE A OF PDB-CODE 1HFB |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.690 |
| Rmerge | 0.067 | 0.330 |
| Number of reflections | 155910 | |
| <I/σ(I)> | 11.8 | 3.5 |
| Completeness [%] | 91.2 | 78.1 |
| Redundancy | 2.1 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.00 |
