1OFJ
RECOMBINANT SPERM WHALE MYOGLOBIN L29H/H64L/D122N MUTANT (WITH INITIATOR MET)
Experimental procedure
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 6 |
Unit cell lengths | 91.442, 91.442, 45.761 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 5.000 - 1.800 |
R-factor | 0.162 |
Rwork | 0.162 |
R-free | 0.21900 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.793 * |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.048 |
Number of reflections | 20475 |
Completeness [%] | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9 | pH 9.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 65 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.4-2.6 (M) | |
3 | 1 | reservoir | Tris-HCl | 20 (mM) | |
4 | 1 | reservoir | EDTA | 1 (mM) |