1OFG
GLUCOSE-FRUCTOSE OXIDOREDUCTASE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04 |
Detector | RIGAKU |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.490, 283.690, 116.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.203 * |
Rwork | 0.203 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 22.900 * |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.090 | 0.265 |
Total number of observations | 353200 * | |
Number of reflections | 71500 | 7701 * |
Completeness [%] | 91.0 | 60 |
Redundancy | 4.8 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 | pH 5.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 5-15 (%(w/v)) | |
3 | 1 | reservoir | succinic acid/KOH | 0.2 (M) | or 0.2 M citric acid/KOH |