1OFF
2Fe-2S Ferredoxin from Synechocystis sp. PCC 6803
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 65 |
Unit cell lengths | 38.788, 38.788, 111.751 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.520 - 1.800 |
R-factor | 0.17553 |
Rwork | 0.174 |
R-free | 0.21600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1czp |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.630 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.500 * | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.055 | 0.317 |
Total number of observations | 161518 * | |
Number of reflections | 8723 | |
Completeness [%] | 98.5 | 97.2 |
Redundancy | 7.7 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * | pH 6.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | HEPES-KOH | 25 (mM) | |
3 | 1 | drop | 100 (mM) | pH7.5 | |
4 | 1 | reservoir | MES-KOH | 100 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 3.6 (M) | |
6 | 1 | reservoir | MPD | 1 (%(v/v)) | pH6.5 |