1OFE
Glutamate Synthase from Synechocystis sp in complex with 2-Oxoglutarate and L-DON at 2.45 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 122.669, 134.377, 198.774 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.450 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1llw |
RMSD bond length | 0.017 |
RMSD bond angle | 1.790 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.800 * | 2.580 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.113 | 0.306 |
Total number of observations | 1066424 * | |
Number of reflections | 120397 | |
Completeness [%] | 99.5 * | 99.9 * |
Redundancy | 2.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | drop | HEPES-KOH | 20 (mM) | |
3 | 1 | drop | glycerol | 10 (%(v/v)) | |
4 | 1 | drop | 2-OG | 2 (mM) | |
5 | 1 | drop | dithiothreitol | 5 (mM) | pH7.5 |
6 | 1 | reservoir | PEG4000 | 20-26 (%(w/v)) | |
7 | 1 | reservoir | Tris-HCl | 100 | |
8 | 1 | reservoir | sodium acetate | 200 (mM) | pH8.0 |