1OFC
nucleosome recognition module of ISWI ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-13 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.470, 66.340, 82.830 |
| Unit cell angles | 90.00, 124.41, 90.00 |
Refinement procedure
| Resolution | 19.000 * - 1.900 |
| R-factor | 0.21946 |
| Rwork | 0.218 |
| R-free | 0.25300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 1.701 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 * | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.300 |
| Total number of observations | 141793 * | |
| Number of reflections | 38275 | 5452 * |
| <I/σ(I)> | 14.8 | 4.1 |
| Completeness [%] | 98.6 | 99.1 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * | 20 * | 1MUL PROTEIN AT 60MG/ML IN 20MM HEPES, 0.5M NACL, PH 7, WAS MIXED WITH 4MUL, 0.125M HEPES PH 7.0, 5% PEG 6000 RESERVOIR 0.1M HEPES, 4% PEG 6000, 0.1M NACL CRYSTALS GROWTH INDUCED BY MICRO SEEDING |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 4 (%) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
| 3 | 1 | drop | Tris-Cl | 50 (mM) | pH7.6 |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | drop | glycerol | 50 (%) |
