1OFC

nucleosome recognition module of ISWI ATPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-13
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	109.470, 66.340, 82.830
Unit cell angles	90.00, 124.41, 90.00

Refinement procedure

Resolution	19.000 * - 1.900
R-factor	0.21946
Rwork	0.218
R-free	0.25300 *
Structure solution method	MAD
RMSD bond length	0.019 *
RMSD bond angle	1.701 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000 *	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.048	0.300
Total number of observations	141793 *
Number of reflections	38275	5452 *
<I/σ(I)>	14.8	4.1
Completeness [%]	98.6	99.1
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *	20 *	1MUL PROTEIN AT 60MG/ML IN 20MM HEPES, 0.5M NACL, PH 7, WAS MIXED WITH 4MUL, 0.125M HEPES PH 7.0, 5% PEG 6000 RESERVOIR 0.1M HEPES, 4% PEG 6000, 0.1M NACL CRYSTALS GROWTH INDUCED BY MICRO SEEDING

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	4 (%)
2	1	reservoir	HEPES	0.1 (M)	pH7.0
3	1	drop	Tris-Cl	50 (mM)	pH7.6
4	1	drop		100 (mM)
5	1	drop	glycerol	50 (%)