1OFB
CRYSTAL STRUCTURE OF THE TYROSINE-REGULATED 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE SYNTHASE FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH MANGANESE(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 2001-07-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 196.636, 50.502, 64.930 |
Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.010 |
Rwork | 0.161 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hfb |
RMSD bond length | 0.023 |
RMSD bond angle | 1.932 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.032 | 0.191 |
Number of reflections | 76999 | |
<I/σ(I)> | 15.98 | 3.82 |
Completeness [%] | 97.4 | 82.7 |
Redundancy | 1.87 | 1.46 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | TRIS PH 7.5-9.0 10 MM, 20% PEG3400, 5% GLYCEROL, 2.4 EQUIV. MN, 13-17MG/ML DAHPS |