1OFA
Crystal structure of the tyrosine-regulated 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from saccharomyces cerevisiae in complex with phosphoenolpyruvate and cobalt(ii)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.210, 50.610, 64.980 |
| Unit cell angles | 90.00, 106.33, 90.00 |
Refinement procedure
| Resolution | 17.570 - 2.020 |
| Rwork | 0.155 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hfb |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.305 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 | 2.150 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.053 | 0.280 |
| Number of reflections | 128877 | |
| <I/σ(I)> | 15.1 | 3.5 |
| Completeness [%] | 96.8 | 86.8 |
| Redundancy | 3.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | TRIS PH 7.5-9.0 10 MM 20% PEG3400, 5% GLYCEROL, 4 EQUIV. PEP, 2.5 EQUIV. CO2+ 13-17MG/ML DAHPS |
