1OF5

Crystal structure of Mex67-Mtr2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	48.820, 83.712, 85.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.242
Rwork	0.242
R-free	0.26200
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.513
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.069	0.370
Number of reflections	9718
<I/σ(I)>	9.4	2
Completeness [%]	95.6	78.5
Redundancy	3.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5	18 *	100MM HEPES PH7.5, PEG 3000 20%, 0.2M CALCIUM ACETATE, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3000	20 (%)
2	1	reservoir	calcium acetate	200 (mM)
3	1	reservoir	HEPES	100 (mM)	pH7.5
4	1	drop	protein	10 (mg/ml)