1OEY
Heterodimer of p40phox and p67phox PB1 domains from human NADPH oxidase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-15 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.97926,0.97912,0.9757959 |
| Spacegroup name | P 64 |
| Unit cell lengths | 151.422, 151.422, 68.317 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 129.000 * - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.155 | 0.473 |
| Total number of observations | 357868 * | |
| Number of reflections | 59062 * | |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 97.7 | 94 |
| Redundancy | 6.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 | 17 * | 18% PEG 3350, 17% PEG 400, 4.8% ISOPROPYL ALCOHOL, 0.1 M CAPSO PH 9.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 10 | 1 | reservoir | PEG400 | 17 (%) | |
| 11 | 1 | reservoir | CAPSO | 0.1 (M) | pH9.0 |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.4 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | PEG3350 | 18 (%) | |
| 6 | 1 | drop | PEG400 | 17 (%) | |
| 7 | 1 | drop | isopropanol | 4.8 (%) | |
| 8 | 1 | drop | CAPSO | 0.1 (M) | pH9.0 |
| 9 | 1 | reservoir | PEG3350 | 18 (%) |
