1OEL

CONFORMATIONAL VARIABILITY IN THE REFINED STRUCTURE OF THE CHAPERONIN GROEL AT 2.8 ANGSTROM RESOLUTION

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	178.380, 204.980, 280.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.800
R-factor	0.227
Rwork	0.227
R-free	0.27000
RMSD bond length	0.008
RMSD bond angle	21.600 *
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.010
High resolution limit [Å]	2.700
Number of reflections	117943
Completeness [%]	88.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	28 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	GroEL	25 (mg/ml)
2	1	drop	PEG8000	0.2 (%(w/v))
3	1	drop	ammonium sulfate	0.86 (M)
4	1	drop		2 (mM)
5	1	drop	Tris-acetate	50 (mM)
6	1	reservoir	PEG8000	0.4 (%(w/v))
7	1	reservoir		4 (mM)
8	1	reservoir	Tris-acetate	100 (mM)