1OE6
Xenopus SMUG1, an anti-mutator uracil-DNA Glycosylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.969, 86.518, 78.456 |
| Unit cell angles | 90.00, 118.53, 90.00 |
Refinement procedure
| Resolution | 43.400 * - 2.650 |
| R-factor | 0.157 |
| Rwork | 0.154 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE STRUCTURE |
| RMSD bond length | 0.051 |
| RMSD bond angle | 4.073 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.400 * | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.092 | 0.438 |
| Number of reflections | 19568 * | |
| <I/σ(I)> | 6.3 | 1.6 |
| Completeness [%] | 99.4 | 96 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 18-25 (%(w/v)) | |
| 2 | 1 | reservoir | isopropanol | 2-12 (%(v/v)) | |
| 3 | 1 | reservoir | HEPES-NaOH | 100 (mM) | pH7 or 8 |
| 4 | 1 | drop | protein | 0.5-7 (mg/ml) |
