1OE5
Xenopus SMUG1, an anti-mutator uracil-DNA Glycosylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.596, 85.653, 78.467 |
| Unit cell angles | 90.00, 118.56, 90.00 |
Refinement procedure
| Resolution | 42.600 * - 2.300 |
| R-factor | 0.166 |
| Rwork | 0.163 |
| R-free | 0.22000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE STRUCTURE |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.568 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 * | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.066 | 0.222 |
| Number of reflections | 29920 | |
| <I/σ(I)> | 6.6 | 3 |
| Completeness [%] | 95.8 | 77.1 |
| Redundancy | 4.0 * | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 18-25 (%(w/v)) | |
| 2 | 1 | reservoir | isopropanol | 2-12 (%(v/v)) | |
| 3 | 1 | reservoir | HEPES-NaOH | 100 (mM) | pH7 or 8 |
| 4 | 1 | drop | protein | 0.5-7 (mg/ml) |
