1OE4
Xenopus SMUG1, an anti-mutator uracil-DNA Glycosylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-15 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.681, 85.936, 79.126 |
Unit cell angles | 90.00, 118.36, 90.00 |
Refinement procedure
Resolution | 69.000 * - 2.000 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.19600 * |
Structure solution method | MIRAS |
RMSD bond length | 0.024 * |
RMSD bond angle | 2.081 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE/RESOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.000 * | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.113 * | 0.289 * |
Number of reflections | 48502 * | |
<I/σ(I)> | 4.7 | 1.3 |
Completeness [%] | 100.0 * | 100 |
Redundancy | 5.4 * | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 18-25 (%(w/v)) | |
2 | 1 | reservoir | isopropanol | 2-12 (%(v/v)) | |
3 | 1 | reservoir | HEPES-NaOH | 100 (mM) | pH7 or 8 |
4 | 1 | drop | protein | 0.5-7 (mg/ml) |