1ODY
HIV-1 PROTEASE COMPLEXED WITH AN INHIBITOR LP-130
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-11 |
| Detector | MACSCIENCE |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.900, 62.900, 83.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| Rwork | 0.167 |
| Structure solution method | RIGID BODY REFINEMENT |
| Starting model (for MR) | 1odx |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.055 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.429 |
| Number of reflections | 12082 | |
| Completeness [%] | 95.0 | 85.1 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 | pH 6.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 15 (%sat) | |
| 2 | 1 | reservoir | sodium citrate | 85 (mM) | |
| 3 | 1 | reservoir | sodium phosphate | 170 (mM) | |
| 4 | 1 | reservoir | MPD | 6 (%) | |
| 5 | 1 | reservoir | sodium azide | 0.02 (%) |
