1ODW

Native HIV-1 Proteinase

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-09-26
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	52.000, 59.400, 62.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.165 *
Rwork	0.165
RMSD bond length	0.021
RMSD bond angle	0.056
Data reduction software	DENZO
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.100
Rmerge	0.069
Total number of observations	30950 *
Number of reflections	10837
Completeness [%]	86.9
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	sodium acetate	50 (mM)
3	1	drop	dithiothreitol	10 (mM)
4	1	drop	inhibitor	20 (mg/ml)	in DMSO
5	1	reservoir	sodium acetate	63 (mM)
6	1	reservoir	sodium phosphate	126 (mM)
7	1	reservoir	ammonium sulfate	37 (%sat)