1ODV

Photoactive yellow protein 1-25 deletion mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Collection date	2000-11-15
Spacegroup name	P 43 21 2
Unit cell lengths	82.566, 82.566, 63.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.000 - 1.140
R-factor	0.1473
R-free	0.17740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2phy
RMSD bond length	0.010
RMSD bond angle	2.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000	1.180
High resolution limit [Å]	1.140	1.140
Rmerge	0.060	0.217
Total number of observations	299780 *
Number of reflections	75208 *
<I/σ(I)>	13.5	4.3
Completeness [%]	94.4	89.2
Redundancy	4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5 *		CRYSTALS WERE GROWN BY EQUILIBRATION OF 1 ML OF 30 MG/ML PROTEIN WITH 1 ML OF MOTHER LIQUOR (1.8 M AMMONIUM SULPHATE, 10 MM COCL[2], 100 MM MES PH 6.5) AGAINST A 1 ML RESERVOIR OF MOTHER LIQUOR. CRYSTALS APPEARED AFTER 2-3 DAYS A LARGEST DIMENSION OF 0.4 MM.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	30 (mg/ml)
2	1	2	ammonium sulfate	1.8 (M)
3	1	2		10 (mM)
4	1	2	MES	100 (mM)