1ODK
PURINE NUCLEOSIDE PHOSPHORYLASE FROM THERMUS THERMOPHILUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-15 |
| Detector | RIGAKU IMAGE PLATE, RAXIS-VII |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 131.919, 131.919, 169.906 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 1.900 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1je1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.800 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.267 |
| Number of reflections | 116626 | |
| <I/σ(I)> | 26 | 4 |
| Completeness [%] | 98.8 | 97.2 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microseeding * | 6.3 | 291 | MICROBATCH METHOD UNDER OIL WAS USED. 15.1 MG/ML OF PROTEIN SOLUTION CONTAINING 0.02M DTT WAS MIXED WITH 1.65M SODIUM ACETATE AND 0.1M MES PH 6.3. THE CRYSTALLIZATION TEMPERATURE WAS 291 K. PARATONE-N OIL MIXED WITH 10% W/W OF GLYCEROL WAS USED FOR CRYOPROTECTION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 0.02 (M) | |
| 3 | 1 | reservoir | sodium acetate | 1.65 (M) | |
| 4 | 1 | reservoir | MES | 0.1 (M) |
