1ODH
Structure of the GCM domain bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.810, 52.910, 62.970 |
Unit cell angles | 90.00, 103.24, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.850 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.28300 * |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.310 * |
Data reduction software | HKL |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.049 | 0.282 * |
Number of reflections | 18243 | |
<I/σ(I)> | 24 | 4 |
Completeness [%] | 97.9 | 95.6 |
Redundancy | 2.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.9 * | pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | drop | 200 (mM) | ||
3 | 1 | drop | Tris | 20 (mM) | pH7.9 |
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | reservoir | PEG6000 | 16-20 (%) | |
6 | 1 | reservoir | MES | 100 (mM) | pH6.0, or 100mM sodium citrate-citric acid pH5.0 |